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A chimeric guide RNA (gRNA) is often used to direct Cas9 protein in the CRISPR system to achieve sequence-specified DNA cleavage. Usually gRNA is produced in vitro by phage polymerases, or in vivo by RNA polymerase III. In this issue, Gao and Zhao (pp. 343C349) report a ribozyme-flanked gRNA based design, allowing gRNA to be produced by other RNA polymerases.
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