Journal of Integrative Plant Biology, 47 (12) : 1500-1506, Dec 2005     Research Article

Transgenic Paulownia Expressing shiva-1 Gene Has Increased Resistance to Paulownia Witches¡¯ Broom Disease

Tao DU, Yao WANG, Qin-Xue HU, Jie CHEN, Sheng LIU, Wen-Jin HUANG and Mu-Lan LIN

Abstract

Stem segments from diseased Paulownia tomentosa¡ÁP. fortunei and leaves from healthy control were transformed with the expression vector p438PRSI via Agrobacterium tumefaciens. The p438PRSI vector contained shiva-1 gene, which encodes an antibacterial peptide under the control of a CaMV35S promoter. The regenerated plants from transformed explants were planted in a greenhouse and nursery. PCR and Southern blotting analysis showed that the shiva-1 gene was successfully integrated into the Paulownia genome. Transcription of the integrated shiva-1 gene was confirmed by RT-PCR. Bioassay in the green house and phytoplasma DNA-dot blotting demonstrated that resistance to Paulownia witch¡¯s broom disease (PWB) increased significantly in shiva-1-transgenic Paulownia. Further investigations indicated that higher Shiva-1 expression correlated with fewer phytoplasma and less symptoms in diseased transgenic Paulownia. Together, our findings strongly suggest that breeding shiva-1-Paulownia is an effective strategy to control PWB disease.
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