Journal of Integrative Plant Biology, 48 (7) : 823-831, July 2006     Research Article


Application of Non-invasive Microsensing System to Simultaneously Measure Both H+ and O2 Fluxes Around the Pollen Tube[OA]

Yue Xu , Tong Sun and Li-Ping Yin


Various ionic and molecular activities in the extracellular environment are vital to plant cell physiological processes. A noninvasive microsensing system (NMS) based on either the scanning ion-selective electrode technique (SIET) or the scanning polarographic electrode technique (SPET) is able to obtain information regarding the transportation of various ions/molecules in intact samples under normal physiological conditions. The two-probe simultaneous test system (2STS) is an integrated system composed of SIET, SPET, and a Xu-Kunkel sampling protocol. In the present study, 2STS was able to simultaneously measure fluxes of H+ and O2 of the lily (Lilium longiflorum Thunb. cv. Ace) pollen tube while avoiding interference between the two probes. The results indicate that the proton fluxes were effluxes, whereas the oxygen fluxes were influxes, and they were closely correlated to each other surrounding the constitutive alkaline band region. Specifically, when the proton effluxes increased, the oxygen influxes also increased. Therefore, the hypothesis of condensed active mitochondria existing in the alkalized area of the pollen tube proposed by Heplerí»s group is supported.(Author for correspondence.E-mail:

© 2017 Institute of Botany, Chinese Academy of Sciences

This Article
  Full Text
  Full Text (PDF)
  Supporting information
  E-mail Link to this Article
  Export Citation for this Article
Citing Articles
  Cited By
Google Scholar
   Articles by Yue Xu
   Articles by Tong Sun
   Articles by Li-Ping Yin
   Articles by Yue Xu
   Articles by Tong Sun
   Articles by Li-Ping Yin
Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail:

Copyright © 2018 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q