Journal of Integrative Plant Biology, 52 (5) : 505ĘC514, Apr 2010     Research Article

Identification of Rice Al-responsive Genes by Semi-quantitative Polymerase Chain Reaction using Sulfite Reductase as a Novel Endogenous Control

Jianjun Zhang1,2, Ying Yin1, Yuqi Wang3 and Xinxiang Peng1,2
1Laboratory of Molecular Plant Physiology, College of Life Sciences, South China Agricultural University; Guangzhou 510642, China
2Key Laboratory of Plant Functional Genomics and Biotechnology, Education Department of Guangdong Province, College of Life Sciences, South China Agricultural University; Guangzhou 510642, China
3Laboratory of Biochemistry and Molecular Biology, College of Life Sciences, South China Agricultural University; Guangzhou 510642, China

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Tel: +86 20 8528 2023; Fax: +86 20 8528 2023; E-mail: xpeng@scau.edu.cn

Online on 16 Mar 2010 at www.jipb.net and www.wileyonlinelibrary.com/journal/jipb
10.1111/j.1744-7909.2010.00931.x

Abstract

Based on the evidence that Al resistance is an inducible process and rice is an Al-resistant crop, identification of Al-responsive genes from rice may help to further clone Al-resistant genes in plants. Semi-quantitative and real-time polymerase chain reaction (PCR) is widely applied in gene transcriptional analyses, particularly for those genes with low transcript abundance. Normalization with proper endogenous control (EC) genes is critical for these two approaches in terms of reliability and precision. We first noticed that the expression of several commonly-used EC genes was depressed under Al stress, while sulfite reductase gene (SR) was stable throughout the Al treatment. The reliability of SR as an EC gene was further tested by analyzing the expression of a number of genes in response to Al challenge. Except for the consistent results obtained for the four previously-identified genes, nine additional genes were newly defined as Al-responsive in this study. Collectively, our results suggest that SR can be used as a novel EC gene for semi-quantitative and real-time PCR analysis of Al responsive genes, and that activated transport of silicon and stimulated metabolism of carotenoid and terpenoid could be involved in Al resistance in rice plants.

Zhang J, Yin Y, Wang Y, Peng X (2010) Identification of rice Al-responsive genes by semi-quantitative polymerase chain reaction using sulfite reductase as a novel endogenous control. J. Integr. Plant Biol. 52(5), 505–514.


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Received 17 Feb 2009   Accepted 20 Dec 2009
© 2017 Institute of Botany, Chinese Academy of Sciences


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