Journal of Integrative Plant Biology, 52 (5) : 496-504, Apr 2010     Research Article

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Reverse Genetic Identification of CRN1 and its Distinctive Role in Chlorophyll Degradation in Arabidopsis[OA]

Guodong Ren1†, Qian Zhou1† , Shouxin Wu1, Yufan Zhang1, Lingang Zhang2, Jirong Huang2, Zhenfei Sun1 and Benke Kuai1
1State Key Laboratory of Genetic Engineering, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200433, China
2National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200032, China
Both authors contributed equally to this paper

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Tel: +86 21 6564 2648; Fax: +86 21 6564 2648; E-mail: bkkuai@fudan.edu.cn

Online on 23 Mar 2010 at www.jipb.net and www.wileyonlinelibrary.com/journal/jipb
10.1111/j.1744-7909.2010.00945.x

Abstract

Recent identification of NYE1/SGR1 brought up a new era for the exploration of the regulatory mechanism of Chlorophyll (Chl) degradation. Cluster analysis of senescence associated genes with putative chloroplast targeting sequences revealed several genes sharing a similar expression pattern with NYE1. Further characterization of available T-DNA insertion lines led to the discovery of a novel stay-green gene CRN1 (Co-regulated with NYE1). Chl breakdown was significantly restrained in crn1-1 under diversified senescence scenarios, which is comparable with that in acd1-20, but much more severe than that in nye1-1. Notably, various Chl binding proteins, especially trimeric LHCP II, were markedly retained in crn1-1 four days after dark-treatment, possibly due to a lesion in disassociation of protein-pigment complex. Nevertheless, the photochemical efficiency of PSII in crn1-1 declined, even more rapidly, two days after dark-treatment, compared to those in Col-0 and nye1-1. Our results suggest that CRN1 plays a crucial role in Chl degradation, and that loss of its function produces various side-effects, including those on the breakdown of Ch-protein complex and the maintenance of the residual photosynthetic capability during leaf senescence.

Ren G, Zhou Q, Wu S, Zhang Y, Zhang L, Huang J, Sun Z, Kuai B (2010) Reverse genetic identification of CRN1 and its distinctive role in chlorophyll degradation in Arabidopsis. J. Integr. Plant Biol. 52(5), 496–504.


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Received 30 Dec 2009   Accepted 10 Jan 2010
© 2017 Institute of Botany, Chinese Academy of Sciences


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