Journal of Integrative Plant Biology, 59 (4) : 275ĘC287, Apr 2017     Research Article


ORA59 and EIN3 interaction couples jasmonate-ethylene synergistic action to antagonistic salicylic acid regulation of PDF expression[OA]

Xiang He†,‡, Jishan Jiang, Changquan Wang§ and Katayoon Dehesh
Department of Botany and Plant Sciences and Institute of Integrative Genome Biology, University of California, Riverside, California 92506, USA

These authors have contributed equally to the manuscript
Present address: Rice Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
§Present address: Department of Ornamental Horticulture, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China

E-mail: Katayoon Dehesh (

Online on 7 Feb 2017 at and


Hormonal crosstalk is central for tailoring plant responses to the nature of challenges encountered. The role of antagonism between the two major defense hormones, salicylic acid (SA) and jasmonic acid (JA), and modulation of this interplay by ethylene (ET) in favor of JA signaling pathway in plant stress responses is well recognized, but the underlying mechanism is not fully understood. Here, we show the opposing function of two transcription factors, ethylene insensitive3 (EIN3) and EIN3-Like1 (EIL1), in SA-mediated suppression and JA-mediated activation of PLANT DEFENSIN1.2 (PDF1.2). This functional duality is mediated via their effect on protein, not transcript levels of the PDF1.2 transcriptional activator octadecanoid-responsive Arabidopsis59 (ORA59). Specifically, JA induces ORA59 protein levels independently of EIN3/EIL1, whereas SA reduces the protein levels dependently of EIN3/EIL1. Co-infiltration assays revealed nuclear co-localization of ORA59 and EIN3, and split-luciferase together with yeast-two-hybrid assays established their physical interaction. The functional ramification of the physical interaction is EIN3-dependent degradation of ORA59 by the 26S proteasome.

These findings allude to SA-responsive reduction of ORA59 levels mediated by EIN3 binding to and targeting of ORA59 for degradation, thus nominating ORA59 pool as a coordination node for the antagonistic function of ET/JA and SA.

Received 26 Jan 2017   Accepted 6 Feb 2017
© 2017 Institute of Botany, Chinese Academy of Sciences

This Article
  Full Text
  Full Text (PDF)
  Supporting information
  E-mail Link to this Article
  Export Citation for this Article
Citing Articles
  Cited By
Google Scholar
   Articles by Xiang He
   Articles by Jishan Jiang
   Articles by Changquan Wang
   Articles by Katayoon Dehesh
   Articles by Xiang He
   Articles by Jishan Jiang
   Articles by Changquan Wang
   Articles by Katayoon Dehesh
Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail:

Copyright © 2018 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q