Journal of Integrative Plant Biology, 59 (7) : 475ĘC495, July 2017     Research Article

OPEN ACCESS ARTICLE

Phenolic metabolism and molecular mass distribution of polysaccharides in cellulose-deficient maize cells[OA]

María de Castro1,2, Romina Martínez-Rubio1, José L. Acebes1, Antonio Encina1, Stephen C. Fry2 and Penélope García-Angulo1
1Área de Fisiología Vegetal. Dpto. Ingeniería y Ciencias Agrarias. Facultad de Biología y Ciencias Ambientales, Universidad de León, Leon E-24071, Spain
2The Edinburgh Cell Wall Group, Institute of Molecular Plant Sciences, School of Biological Sciences, Edinburgh EH9 3BF, UK

Correspondences:
E-mail: Penélope García-Angulo (pgara@unileon.es)

Online on 5 May 2017 at www.jipb.net and www.wileyonlinelibrary.com/journal/jipb

Abstract

As a consequence of the habituation to low levels of dichlobenil (DCB), cultured maize cells presented an altered hemicellulose cell fate with a lower proportion of strongly wall-bound hemicelluloses and an increase in soluble extracellular polymers released into the culture medium. The aim of this study was to investigate the relative molecular mass distributions of polysaccharides as well as phenolic metabolism in cells habituated to low levels of DCB (1.5 μM). Generally, cell wall bound hemicelluloses and sloughed polymers from habituated cells were more homogeneously sized and had a lower weight-average relative molecular mass. In addition, polysaccharides underwent massive cross-linking after being secreted into the cell wall, but this cross-linking was less pronounced in habituated cells than in non-habituated ones. However, when relativized, ferulic acid and p-coumaric acid contents were higher in this habituated cell line. Feasibly, cells habituated to low levels of DCB synthesized molecules with a lower weight-average relative molecular mass, although cross-linked, as a part of their strategy to compensate for the lack of cellulose.

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Received 30 Mar 2017   Accepted 21 Apr 2017
© 2017 Institute of Botany, Chinese Academy of Sciences


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