Journal of Integrative Plant Biology, 59 (9) : 680ĘC692, Sept 2017     Research Article

OPEN ACCESS ARTICLE

ZYGOTE-ARREST 3 that encodes the tRNA ligase is essential for zygote division in Arabidopsis[OA]

Ke-Jin Yang1,2,3†, Lei Guo1,2†, Xiu-Li Hou1,2, Hua-Qin Gong1 and Chun-Ming Liu1,4
1Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
2University of Chinese Academy of Sciences, Beijing 100049, China
3School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China
4Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China

These authors contributed equally to this work.

Correspondence:
E-mail: Chun-Ming Liu (cmliu@ibcas.ac.cn)

Online on 20 June 2017 at www.jipb.net and www.wileyonlinelibrary.com/journal/jipb

Abstract

In sexual organisms, division of the zygote initiates a new life cycle. Although several genes involved in zygote division are known in plants, how the zygote is activated to start embryogenesis has remained elusive. Here, we showed that a mutation in ZYGOTE-ARREST 3 (ZYG3) in Arabidopsis led to a tight zygote-lethal phenotype. Map-based cloning revealed that ZYG3 encodes the transfer RNA (tRNA) ligase AtRNL, which is a single-copy gene in the Arabidopsis genome. Expression analyses showed that AtRNL is expressed throughout zygotic embryogenesis, and in meristematic tissues. Using pAtRNL::cAtRNL-sYFP-complemented zyg3/zyg3 plants, we showed that AtRNL is localized exclusively in the cytoplasm, suggesting that tRNA splicing occurs primarily in the cytoplasm. Analyses using partially rescued embryos showed that mutation in AtRNL compromised splicing of intron-containing tRNA. Mutations of two tRNA endonuclease genes, SEN1 and SEN2, also led to a zygote-lethal phenotype. These results together suggest that tRNA splicing is critical for initiating zygote division in Arabidopsis.


---------------------------------------------------
Received 25 Apr 2017   Accepted 16 June 2017
© 2017 Institute of Botany, Chinese Academy of Sciences


This Article
  Full Text
  Full Text (PDF)
  Supporting information
Services
  E-mail Link to this Article
  Export Citation for this Article
Citing Articles
  Cited By
Google Scholar
   Articles by Ke-Jin Yang
   Articles by Lei Guo
   Articles by Xiu-Li Hou
   Articles by Hua-Qin Gong
   Articles by Chun-Ming Liu
PubMed
   Articles by Ke-Jin Yang
   Articles by Lei Guo
   Articles by Xiu-Li Hou
   Articles by Hua-Qin Gong
   Articles by Chun-Ming Liu
Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn

Copyright © 2017 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q