Journal of Integrative Plant Biology, 59 (9) : 629ĘC641, Sept 2017     Research Article

OPEN ACCESS ARTICLE

SNAIL1 is essential for female gametogenesis in Arabidopsis thaliana[OA]

Lihong Hao1†, Xiaolin Wei1†, Jiulei Zhu1, Jiao Shi1, Jingjing Liu1,2, Hongya Gu1,2, Tomohiko Tsuge3 and Li-Jia Qu1,2
1State Key Laboratory for Protein and Plant Gene Research, Peking-Tsinghua Center for Life Sciences, School of Life Sciences, Peking University, Beijing 100871, China
2National Plant Gene Research Center (Beijing), Beijing 100101, China
3Institute for Chemical Research, Kyoto University, Gokasho Uji, Kyoto 611-0011, Japan

These authors have contributed equally to the manuscript
Correspondences:
E-mail: Li-Jia Qu (qulj@pku.edu.cn, Dr. Qu is fully responsible for distributions of all materials associated with this article); Tomohiko Tsuge (tsuge@scl.kyoto-u.ac.jp)

Online on 4 Aug 2017 at www.jipb.net and www.wileyonlinelibrary.com/journal/jipb

Abstract

Two yeast Brix family members Ssf1 and Ssf2, involved in large ribosomal subunit synthesis, are essential for yeast cell viability and mating efficiency. Their putative homologs exist in the Arabidopsis genome; however, their role in plant development is unknown. Here, we show that Arabidopsis thaliana SNAIL1 (AtSNAIL1), a protein sharing high sequence identity with yeast Ssf1 and Ssf2, is critical to mitosis progression of female gametophyte development. The snail1 homozygous mutant was nonviable and its heterozygous mutant was semi-sterile with shorter siliques. The mutation in SNAIL1 led to absence of female transmission and reduced male transmission. Further phenotypic analysis showed that the synchronic development of female gametophyte in the snail1 heterozygous mutant was greatly impaired and the snail1 pollen tube growth, in vivo, was also compromised. Furthermore, SNAIL1 was a nucleolar-localized protein with a putative role in protein synthesis. Our data suggest that SNAIL1 may function in ribosome biogenesis like Ssf1 and Ssf2 and plays an important role during megagametogenesis in Arabidopsis.


---------------------------------------------------
Received 22 May 2017   Accepted 2 Aug 2017
© 2017 Institute of Botany, Chinese Academy of Sciences


This Article
  Full Text
  Full Text (PDF)
  Supporting information
Services
  E-mail Link to this Article
  Export Citation for this Article
Citing Articles
  Cited By
Google Scholar
   Articles by Lihong Hao
   Articles by Xiaolin Wei
   Articles by Jiulei Zhu
   Articles by Jiao Shi
   Articles by Jingjing Liu
   Articles by Hongya Gu
   Articles by Tomohiko Tsuge
   Articles by Li-Jia Qu
PubMed
   Articles by Lihong Hao
   Articles by Xiaolin Wei
   Articles by Jiulei Zhu
   Articles by Jiao Shi
   Articles by Jingjing Liu
   Articles by Hongya Gu
   Articles by Tomohiko Tsuge
   Articles by Li-Jia Qu
Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn

Copyright © 2017 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q