Journal of Integrative Plant Biology, 60 (5) : 369-375, May 2018     Research Article

OPEN ACCESS ARTICLE

Generation of new glutinous rice by CRISPR/Cas9坼targeted mutagenesis of the Waxy gene in elite rice varieties[OA]

Jinshan Zhang1,2†, Hui Zhang1,3†, Jos谷 Ram車n Botella4 and Jian-Kang Zhu1,3
1Shanghai Center for Plant Stress Biology, and Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 201602, China
2University of Chinese Academy of Sciences, Shanghai 201602, China
3Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USA
4Plant Genetic Engineering Laboratory, School of Agriculture and Food Sciences, University of Queensland, Brisbane QLD 4072, Australia

These authors contributed equally to this work.

Correspondences:
Email: Hui Zhang (huizhang@sibs.ac.cn); Jian-Kang Zhu (jkzhu@sibs.ac.cn, Dr. Zhu is fully responsible for the distribution of all materials associated with this article)

Online on 6 Dec 2017 at www.jipb.net and www.wileyonlinelibrary.com/journal/jipb

Abstract

In rice, amylose content (AC) is controlled by a single dominant Waxy gene. We used Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR坼associated 9 (Cas9) to introduce a loss坼of坼function mutation into the Waxy gene in two widely cultivated elite japonica varieties. Our results show that mutations in the Waxy gene reduce AC and convert the rice into glutinous ones without affecting other desirable agronomic traits, offering an effective and easy strategy to improve glutinosity in elite varieties. Importantly, we successfully removed the transgenes from the progeny. Our study provides an example of generating improved crops with potential for commercialization, by editing a gene of interest directly in elite crop varieties.


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Received 23 Nov 2017   Accepted 1 Dec 2017
© 2017 Institute of Botany, Chinese Academy of Sciences


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