Journal of Integrative Plant Biology, 60 (6) : 498-513, June 2018     Research Article

OPEN ACCESS ARTICLE

Silencing GRAS2 reduces fruit weight in tomato[OA]

Miao Li, Xin Wang, Changxing Li, Hanxia Li, Junhong Zhang and Zhibiao Ye
Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China

Correspondence:
Email: Junhong Zhang (zhangjunhng@mail.hzau.edu.cn)

Online on 23 Jan 2018 at www.jipb.net and www.wileyonlinelibrary.com/journal/jipb

Abstract

GRAS family transcription factors are involved in multiple biological processes in plants. Here, we report that GRAS2 plays a vital role in regulating fruit weight in tomato (Solanum lycopersicum). We establish that the expression of GRAS2 was elevated in ovaries and maintained at a constant level in fertilized ovules. Reduction of GRAS2 expression in transgenic plants reduced fruit weight through modulating ovary growth and cell size. At the metabolic level, downregulation of GRAS2 decreased activities of the gibberellic acid biosynthesis and signal transduction pathways, leading to insufficient levels of active gibberellic acid during the initial ovary development of tomato. Moreover, genotypic diversity of GRAS2 was consistent with the molecular basis of fruit weight evolution, suggesting that GRAS2 contributes to the molecular basis of the evolution of fruit weight in tomato. Collectively, these findings enhance our understanding of GRAS2 functions, in fruit development of tomato, and demonstrate a strong association between the GRAS gene family and fruit development.


---------------------------------------------------
Received 30 Oct 2017   Accepted 9 Jan 2018
© 2017 Institute of Botany, Chinese Academy of Sciences


This Article
  Full Text
  Full Text (PDF)
  Supporting information
Services
  E-mail Link to this Article
  Export Citation for this Article
Citing Articles
  Cited By
Google Scholar
   Articles by Miao Li
   Articles by Xin Wang
   Articles by Changxing Li
   Articles by Hanxia Li
   Articles by Junhong Zhang
   Articles by Zhibiao Ye
PubMed
   Articles by Miao Li
   Articles by Xin Wang
   Articles by Changxing Li
   Articles by Hanxia Li
   Articles by Junhong Zhang
   Articles by Zhibiao Ye
Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn

Copyright © 2018 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q