Journal of Integrative Plant Biology, 60 (8) : 670-690, Aug 2018     Research Article

OPEN ACCESS ARTICLE

Characterization of maize leaf pyruvate orthophosphate dikinase using high throughput sequencing[OA]

Yuling Zhang1, Rita Giuliani2, Youjun Zhang3, Yang Zhang4, Wagner Luiz Araujo3, Baichen Wang5, Peng Liu6, Qi Sun7, Asaph Cousins2, Gerald Edwards2, Alisdair Fernie3, Thomas P. Brutnell8 and Pinghua Li1
1State Key Laboratory of Crop Biology, College of Agronomic Sciences, Shandong Agricultural University, Tai¡¯an 271018, China
2School of Biological Sciences, Molecular Plant Sciences, Washington State University, Pullman, WA 99164-4236, USA
3Max-Planck-Insitut f¨¹r Molekulare Pflanzenphysiologie, Am M¨¹hlenberg 1, Potsdam-Golm D-14476, Germany
4Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Nebraska, USA
5Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, No.20 Nanxincun, Xiangshan, Beijing 100093, China
6Department of Statistics, Iowa State University, Ames, Iowa 50011, USA
7Computational Biology Service Unit, Life Sciences Core Laboratories Center, Cornell University, Ithaca, New York 14850, USA
8Donald Danforth Plant Science Center, St. Louis, Missouri 63132, USA

Correspondence:
Email: Pinghua Li (pinghuali@sdau.edu.cn)

Online on 17 Apr 2018 at www.jipb.net and www.wileyonlinelibrary.com/journal/jipb

Abstract

In C4 photosynthesis, pyruvate orthophosphate dikinase (PPDK) catalyzes the regeneration of phosphoenolpyruvate in the carbon shuttle pathway. Although the biochemical function of PPDK in maize is well characterized, a genetic analysis of PPDK has not been reported. In this study, we use the maize transposable elements Mutator and Ds to generate multiple mutant alleles of PPDK. Loss©\of©\function mutants are seedling lethal, even when plants were grown under 2% CO2, and they show very low capacity for CO2 assimilation, indicating C4 photosynthesis is essential in maize. Using RNA©\seq and GC©\MS technologies, we examined the transcriptional and metabolic responses to a deficiency in PPDK activity. These results indicate loss of PPDK results in downregulation of gene expression of enzymes of the C4 cycle, the Calvin cycle, and components of photochemistry. Furthermore, the loss of PPDK did not change Kranz anatomy, indicating that this metabolic defect in the C4 cycle did not impinge on the morphological differentiation of C4 characters. However, sugar metabolism and nitrogen utilization were altered in the mutants. An interaction between light intensity and genotype was also detected from transcriptome profiling, suggesting altered transcriptional and metabolic responses to environmental and endogenous signals in the PPDK mutants.


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Received 18 Mar 2018   Accepted 12 Apr 2018
© 2017 Institute of Botany, Chinese Academy of Sciences


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   Articles by Yuling Zhang
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   Articles by Baichen Wang
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   Articles by Alisdair Fernie
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   Articles by Pinghua Li
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