Journal of Integrative Plant Biology, 60 (8) : 626-631, Aug 2018     Research Article

OPEN ACCESS ARTICLE

Multiplex gene editing in rice with simplified CRISPRİ\Cpf1 and CRISPRİ\Cas9 systems[OA]

Mugui Wang1, Yanfei Mao1, Yuming Lu1, Zhidan Wang1, Xiaoping Tao1 and Jian-Kang Zhu1,2
1Shanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 201602, China
2Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USA

Correspondences:
Email: Mugui Wang (mgwang@sibs.ac.cn), Jian-Kang Zhu (jkzhu@sibs.ac.cn,
Dr. Zhu is fully responsible for the distribution of all materials associated with this article.)

Online on 15 May 2018 at www.jipb.net and www.wileyonlinelibrary.com/journal/jipb

Abstract

We developed simplified single transcriptional unit (SSTU) CRISPR systems for multiplex gene editing in rice using FnCpf1, LbCpf1 or Cas9, in which the nuclease and its crRNA array are coİ\expressed from a single Pol II promoter, without any additional processing machinery. Our SSTU systems are easy to construct and effective in mediating multiplex genome editing.


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Received 23 Mar 2018   Accepted 10 May 2018
© 2017 Institute of Botany, Chinese Academy of Sciences


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