Acta Botanica Sinica Volume 44 Issue 3, Pages 373-376.
Location and Analysis of Introgressed Segments in the Parthenogenetic Progenies of Zea mays×Z.diploperennis by GISH

WEI Wen Hui, QIN Rui, SONG Yun Chun*, NING Shun Bin, GUO Le Qun, GU Ming Guang

Abstract:

玉米×二倍体多年生类玉米孤雌后代渗入片段的GISH定位分析

魏文辉1  覃瑞1  宋运淳1*  宁顺斌1  郭乐群2  谷明光2

(1.武汉大学植物发育生物学教育部重点实验室,武汉,4300722.中国科学院遗传与发育生物学研究所,北京,100101)

摘要:用来自玉米(Zea mays L.)与二倍体多年生类玉米(Z. diploperennis Iltis, Doebley and Guzmán)杂交的孤雌生殖后代同一抗病个体的4个株系进行了基因组原位杂交,用改进的杂交技术获得了近100%的检出率,每一检出片段在同源染色体两成员和每两个姊妹染色单体上均有清晰的信号.

关键词 玉米 二倍体多年生类玉米 渗入片段 基因组原位杂交

*通讯作者 Email: ycsong@whu.edu.cn

 


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