Acta Botanica Sinica Volume 36 Issue 3, Pages .
Marker Proteins for Somatic Embryogenesis in Several Plants

Zheng Xiao-feng and Huang Bai-qu

Abstract:
SDS polyacrylamide gel electrophoresis was employed to examine the soluble protein composition of embryogenic callus, non-embryogenic callus, as well as developing embryoids in Panax quinquefolius L., Freesia refracta Klatt., Oxytropis leptophylla L. and Medicago suaveolens L. The results showed that embryogenic proteins were specifically present in potentially embryogenic calluses in all the 4 plant species studied. In Panax quinquefolius and Oxytropis leptophylla , the same embryogenic proteins were detected in developing embryoids. Besides a number of callus-specfic proteins was also found in non-embryogenic calluses of Freesia refracta and Medicago suaveolens. Although diverse in number, molecular weight and life span in different species, these specfic proteins seem to be related to the embryogenic state of callus, and may be used as markers to identify potentially embryogenic cultures and to trace the development of embryoids. They also provide the basis for studies of gene activities and molecular mechanisms of somatic embryogenesis in these plant species.

Keywords:
Embryogenic calluses; Non-embryogenic calluses; Marker proteins;Panax quinquefolius; Freesia refracta; Oxytropis leptophylla; Medicago suaveolens
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