Acta Botanica Sinica Volume 39 Issue 5, Pages .
Geographical Variation in the Characteristics of Absorption Spectra and Fluorescence Spectra of Pinus armandi

Tang Li-jun, Li Bo-sheng, Tang Chong-qin, Kuang Ting-yun and Tang Pei-song

Abstract:
By measuring the derivative absorption spectra of chloroplasts of different populations of Pinus armandi Franch., it was found that southern populations maintained higher absorption at 680 nm than at 670 nm, but some of the northern populations deviated the maximum absorption from 680 nm to 670 nm, which indicated that the activity of PS Άς in some of the northern populations declined. Clear geographical differences also have been found in the positions of emission peaks of PS Άρ and PS Άς in the fluorescence emission spectra at 77 K. Analysis of the fluorescence excitation spectra at 77 K revealed geographical changes in the absorption.status of Chl a. Besides, the experimental results indicated that the intact needles of Pinus armandi are not ideal materials to be used in detecting the geographical variation in photochemical reaction process because the presence of thicker coat, resin etc. can conceal the spectral differences in different populations.

Keywords:
Pinus armandi, Geographical variation, Fourth derivative absorption spectra, Fluorescence emission spectra at 77 K, Fluorescence excitation spectra at 77 K
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