Overexpression of OsPAP10a, A Root-Associated Acid Phosphatase, Increased Extracellular Organic Phosphorus Utilization in Rice

doi:10.1111/j.1744-7909.2012.01143.x

J Integr Plant Biol. ›› 2012, Vol. 54 ›› Issue (9): 631-639.DOI: 10.1111/j.1744-7909.2012.01143.x

• Plant-environmental Interactions • Previous Articles Next Articles

Overexpression of OsPAP10a, A Root-Associated Acid Phosphatase, Increased Extracellular Organic Phosphorus Utilization in Rice

Jingluan Tian^1,2†, Chuang Wang^1,2†, Qian Zhang^1‡, Xiaowei He¹, James Whelan^1,3 and Huixia Shou^1,2*

¹State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, P.R. China
²Joint Research Laboratory in Genomics and Nutriomics, College of Life Sciences, Zhejiang University, Hangzhou 310058, P. R. China
³Australian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley 6009, WA, Australia

Received:2012-04-22 Accepted:2012-07-05 Published:2012-07-16
About author:^‡Present address: Guangdong Academy of Forestry, Guangzhou 510520, P.R. China.
^†These authors contributed equally to this work.
^*Corresponding author: Tel/Fax +86 571 88206146; E-mail: huixia@zju.edu.cn

Abstract

Abstract:

Phosphorus (P) deficiency is a major limitation for plant growth and development. Among the wide set of responses to cope with low soil P, plants increase their level of intracellular and secreted acid phosphatases (APases), which helps to catalyze inorganic phosphate (Pi) hydrolysis from organo-phosphates. In this study we characterized the rice (Oryza sativa) purple acid phosphatase 10a (OsPAP10a). OsPAP10a belongs to group Ia of purple acid phosphatases (PAPs), and clusters with the principal secreted PAPs in a variety of plant species including Arabidopsis. The transcript abundance of OsPAP10a is specifically induced by Pi deficiency and is controlled by OsPHR2, the central transcription factor controlling Pi homeostasis. In gel activity assays of root and shoot protein extracts, it was revealed that OsPAP10a is a major acid phosphatase isoform induced by Pi starvation. Constitutive overexpression of OsPAP10a results in a significant increase of phosphatase activity in both shoot and root protein extracts. In vivo root 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) assays and activity measurements on external media showed that OsPAP10a is a root-associated APase. Furthermore, overexpression of OsPAP10a significantly improved ATP hydrolysis and utilization compared with wild type plants. These results indicate that OsPAP10a can potentially be used for crop breeding to improve the efficiency of P use.

Tian J, Wang C, Zhang Q, He X, Whelan J, Shou H (2012) Overexpression of OsPAP10a, a root-associated acid phosphatase, increased extracellular organic phosphorus utilization in rice. J. Integr. Plant Biol. 54(9), 631–639.

Key words: Purple acid phosphatase, phosphate, rice, OsPAP10a, root-associated APase

Jingluan Tian, Chuang Wang, Qian Zhang, Xiaowei He, James Whelan, and Huixia Shou. Overexpression of OsPAP10a, A Root-Associated Acid Phosphatase, Increased Extracellular Organic Phosphorus Utilization in Rice[J]. J Integr Plant Biol., 2012, 54(9): 631-639.

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Overexpression of OsPAP10a, A Root-Associated Acid Phosphatase, Increased Extracellular Organic Phosphorus Utilization in Rice

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