J Integr Plant Biol. ›› 2017, Vol. 59 ›› Issue (1): 60-74.DOI: 10.1111/jipb.12505

• New Resources • Previous Articles    

Development and utilization of a new chemically-induced soybean library with a high mutation density

Zhongfeng Li1, Lingxue Jiang1, Yansong Ma1,2, Zhongyan Wei1, Huilong Hong1, Zhangxiong Liu1, Jinhui Lei1, Ying Liu1, Rongxia Guan1, Yong Guo1, Longguo Jin1, Lijuan Zhang1, Yinghui Li1, Yulong Ren1, Wei He1, Ming Liu1, Nang Myint Phyu Sin Htwe1, Lin Liu1, Bingfu Guo1, Jian Song1, Bing Tan1, Guifeng Liu1, Maiquan Li1, Xianli Zhang1, Bo Liu1, Xuehui Shi1, Sining Han1, Sunan Hua1, Fulai Zhou1, Lili Yu1, Yanfei Li1, Shuang Wang1, Jun Wang1,3, Ruzhen Chang1 and Lijuan Qiu1*   

  1. 1National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China
    2Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China
    3College of Agriculture, Yangtze University, Jingzhou 434025, China
  • Received:2016-08-15 Accepted:2016-10-20 Published:2016-10-24
  • About author:*Correspondence: E-mail: Lijuan Qiu (qiulijuan@caas.cn)

Abstract:

Mutagenized populations have provided important materials for introducing variation and identifying gene function in plants. In this study, an ethyl methanesulfonate (EMS)-induced soybean (Glycine max) population, consisting of 21,600 independent M2 lines, was developed. Over 1,000 M4 (5) families, with diverse abnormal phenotypes for seed composition, seed shape, plant morphology and maturity that are stably expressed across different environments and generations were identified. Phenotypic analysis of the population led to the identification of a yellow pigmentation mutant, gyl, that displayed significantly decreased chlorophyll (Chl) content and abnormal chloroplast development. Sequence analysis showed that gyl is allelic to MinnGold, where a different single nucleotide polymorphism variation in the Mg-chelatase subunit gene (ChlI1a) results in golden yellow leaves. A cleaved amplified polymorphic sequence marker was developed and may be applied to marker-assisted selection for the golden yellow phenotype in soybean breeding. We show that the newly developed soybean EMS mutant population has potential for functional genomics research and genetic improvement in soybean.

Key words: EMS mutagenesis, mutant population, soybean, whole-genome resequencing

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