J Integr Plant Biol. ›› 2000, Vol. 42 ›› Issue (1): 105-106.

• Research Articles • Previous Articles     Next Articles

An Approach to mRNA Whole-mount in Situ Hybridization for Plant Young Embryos

LI Shi-Tao, Lü Ying-Tang, YANG Hong-Yuan   

Abstract:

A mRNA whole-mount in situ hybridization method is reported here for quick, direct analysis of the spatial and temporal mRNA expression patterns in plant young embryos. A cDNA clone THE3 (tobacco heart embryo 3) was isolated by differential screening from tobacco (Nicotiana tabacum L.) heart embryo cDNA library as compared with the globular embryo cDNA library. The distribution of THE3 mRNA in tobacco heart embryos and globular embryos was investigated by a whole-mount in situ hybridization technique, showing that THE 3 is preferentially expressed in heart embryos.

一种适于植物幼胚mRNA整体原位杂交的方法
李师弢 吕应堂 杨弘远*

(武汉大学生命科学学院发育生物学研究中心,武汉430072)


关键词: 幼胚;mRNA原位杂交;烟草

Key words: embryo, mRNA in situ hybridization, Nicotiana tabacum

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
备案号:京ICP备16067583号-22