J Integr Plant Biol. ›› 2000, Vol. 42 ›› Issue (3): 239-243.
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WANG Wei-Jun, LIN Jian-Qiao, LI Ming-Qi
Abstract:
The binding condition between flavin mononucleotide (FMN) and protein moiety of glycollate oxidase (GO) was investigated in 11 species of higher plants from 7 families. A loose binding of FMN with GO was universally observed in all the plants. According to this character, a new efficient but mild method was established for preparation of FMN-free GO, which could produce 87.5% recovery of activity . FMN-free GO could be reactivated by adding FMN. For half reactivation 8×10 mol/L FMN was needed, and more than 5×10 mol/L for 100%. The result indicates that there exists a reversible dissociation balance between FMN and protein moiety of GO. Therefore, the concentration of FMN may act as a factor to regulate GO activity in higher plants.
高等植物乙醇酸氧化酶与黄素单核苷酸的松弛结合 王炜军 林健巧 李明启 (华南农业大学生物技术学院,广州510642)
摘要:普查了 7个科 11种植物的乙醇酸氧化酶 (GO) ,发现其酶蛋白与黄素单核苷酸 (FMN)的结合均是松弛的 ;并据此特征找到了一种温和的制备完全脱FMNGO的新方法 ,酶液总活性回收可达 87.5 % ;外加FMN可使脱辅因子GO不同程度地恢复活性 ,恢复 5 0 %活性所需FMN的浓度为 8× 10 -7mol/L ,而当浓度大于 5× 10 -6mol/L时其复活作用达到 10 0 % ,表明两者间存在一个可逆的解离平衡。推测植物体内的FMN浓度可能是乙醇酸氧化酶活性的一个调节因子。
关键词: 乙醇酸氧化酶;黄素单核苷酸;解离平衡;高等植物
Key words: glycollate oxidase, flavin mononucleotide, balance of dissociation, higher plants
WANG Wei-Jun, LIN Jian-Qiao, LI Ming-Qi. Binding Between Flavin Mononucleotide and Glycollate Oxidase from Higher Plants[J]. J Integr Plant Biol., 2000, 42(3): 239-243.
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