J Integr Plant Biol. ›› 2001, Vol. 43 ›› Issue (3): 312-315.

• Research Articles • Previous Articles     Next Articles

Isolation and Structural Determination of a Glucan from the Spores of Ganoderma lucidum

BAO Xing-Feng, FANG Ji-Nian   

Abstract:

A water soluble, (1→6)-branched, (1→4)linked D-glucan (LB-B1),[α]21D=+174.2° (c 0.87, H2O), was obtained from a hot-water extract of the sporoderm-broken spores of Ganoderma lucidum (Fr.) Karst by HPSEC, with 0.001 mol/L sodium hydroxide as the eluant, the molecular weight ( Mw ) of LB-B1 was estimated to be 9.3×103. From the results of total hydrolysis, methylation analysis, acetolysis and 1D, 2DNMR experimentation, it was concluded that LB-B1 was composed of repeating units with the following structure:α-D-Glcp-(1→6)-α-D -Glcp-(1→6)-α-D-Glcp 1↓6→4)-α- D -Glcp-(1→4)-α- D -Glcp-(1→4)-α- D -Glcp-(1→4)-α-D-Glcp-(1→4-)-α-D-Glcp-(1→)n

赤芝孢子粉中一个葡聚糖的分离纯化与结构鉴定
鲍幸峰 方积年*

(中国科学院上海药物研究所,上海200031)

摘要:从破壁的赤芝(Ganodermalucidum(Fr.)Karst)孢子粉中分离纯化到一个水溶性的、以1,4连接的D-葡萄糖为主链、在6位接1,6连接葡萄糖的α-D-葡聚糖。高效分子排阻色谱测定,重均分子量为9.3×103,[α]21D=+174.2°(c0.87,H2O)。通过糖组成分析,甲基化反应,乙酰解及一维和二维核磁等光谱解析,确定其结构如下:

关键词: 葡聚糖;多糖;破壁赤芝孢子粉

Key words: glucan, polysaccharide, sporoderm-broken spores of Ganoderma lucidum

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