Abstract:

Three deletion mutants of tobacco mosaic virus (TMV) 54-kD putative replicase gene (54K) were obtained by PCR, and cloned into plant expression vector p208, then transformed into Nicotiana tabacum L. cv. SR1 by Agrobacterium tumefaciens (Smith et Townsend) Conn Ti plasmid-mediated transformation. All the transgenic plants with the N-terminal deletion mutant, the C-terminal deletion mutant and the only 261 nucleotides region from the central part of the 54K ORF showed significant resistance against TMV. 

TMV 54K基因的3 个突变体介导抗病性的研究
安利忻 刘一飞 陈章良 李 毅*

（北京大学生命科学学院蛋白质工程和植物基因工程国家重点实验室，北大!耶鲁植物分子遗传农业生物技术联合中心，北京100871）

摘要：利用PCR方法分别构建了烟草花叶病毒(TMV)中一个推测为复制酶的54-kD蛋白基因(54K)缺失N端、C端和仅余基因中部261bp的3个缺失突变体，与野生型54K一起克隆入植物中间载体p208，并通过根癌土壤杆菌(Agrobacteriumtumefaciens (Smith et Townsend) Conn)介导的方法转化烟草(Nicotiana tabacum L.)。用TMV侵染转基因植物的R0代和R1代，结果显示这3个缺失突变体均能介导对TMV的抗病性。

关键词：TMV ;54K突变体；复制酶介导的抗病性

Key words: TMV, 54K mutants, replicase-mediated resistance