J Integr Plant Biol. ›› 2001, Vol. 43 ›› Issue (8): 874-876.

• Research Articles • Previous Articles     Next Articles

Cloning and Sequencing the Full-length cDNA of Annexin from Strawberry Fruit

WANG Guan-Lin, YANG Huai-Yi*, XIA Ran, FANG Hong-Jun, JING Shi-Xi   

Abstract:

草莓果实膜联蛋白基因(annfaf)全长cDNA克隆及序列分析
王关林1  杨怀义1,2*  夏然1 方宏筠1  景士西3

(1.辽宁师范大学生命科学学院,大连116029;2.  中国科学院微生物研究所,北京100081;3. 沈阳农业大学园艺系,沈阳110161)

摘要:采用RACE技术从草莓(Fragaria ananassa Duch.)成熟果实中分离克隆了膜联蛋白(annexin)基因的cDNA 5′-端未知序列,并通过测序确定了翻译的起始密码位点、终止密码位点及完整的读码框,从而首次获得了草莓果实膜联蛋白基因的cDNA全序列,命名为annfaf(annexin of Fragaria ananassa fruit)基因.

关键词: 草莓;果实膜联蛋白;cDNA 克隆;annfaf(annexin of Fragaria ananassa fruit)基因

Key words: strawberry, annexin protein of fruit, cDNA clone, annfaf (annexin of Fragaria ananassa fruit) gene

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