J Integr Plant Biol. ›› 2001, Vol. 43 ›› Issue (8): 877-880.

• Research Articles • Previous Articles    

Chloroplast Composition and Structural Differences in a Chlorophyll-reduced Mutant of Oilseed Rape Seedlings

ZHAO Yun, DU Lin-Fang, YANG Sheng-Hong, LI Shi-Chong, ZHANG Yi-Zheng   

Abstract:

缺乏叶绿素的油菜突变体的叶绿体组成和结构变化
赵云 杜林方* 杨胜洪 李世崇 张义正
(四川大学生命科学学院,成都610064)

摘要:对黄化突变体Cr3529和野生型油菜(Brassica napus L.) 3529叶绿体的超微结构和组成进行了比较.与野生型相比, 突变体Cr3529叶片具有较少的类囊体、较少的垛叠膜区和较少的叶绿素含量.突变体的Chl a/Chl b比值较高, 是野生型的2倍.电泳结果表明,突变体类囊体膜中LHCⅡ和其三聚体LHCⅡ*的含量减少.SDS-PAGE分析显示,LHCⅡ的脱辅基蛋白在突变体类囊体膜中明显减少.免疫印迹进一步表明,所有LHCⅡ组分的含量仅为野生油菜的类囊体膜的1/3.突变体Cr3529的天线系统比野生型3529的小.

关键词: 油菜;类囊体膜;色素蛋白;叶绿素减少突变体

Key words: oilseed rape, thylakoid membrane, pigment protein, chlorophyll-reduced mutant

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