Impact of Copy Number on Transgene Expression In Tobacco

Abstract

Abstract:

GUS基因拷贝数对转基因在受体植物烟草中表达的影响

李旭刚陈松彪路子显常团结曾千春朱祯^*

(中国科学院遗传研究所,北京 100101)

摘要：对农杆菌介导法获得的转β-葡糖醛酸酶(β-glucuronidase, GUS)基因(uidA)烟草(Nicotiana tabacum L.)进行GUS表达分析,发现部分转基因植株无GUS活性.进一步Southern杂交结果发现,GUS基因失活植株的基因组中整合了多个uidA拷贝,而GUS活性高的转基因植株多为uidA单拷贝整合,表明uidA基因失活与基因多拷贝整合有关. Northern杂交结果显示,失活植株无特异uidA RNA杂交带,而GUS活性高的植株可检测到明显的杂交信号,说明多拷贝引起的基因失活发生在RNA水平.

关键词转基因植物 uidA基因多拷贝整合基因失活

*通讯作者 E-mail: zzhu@genetics.ac.cn

Key words: transgenic plant, uidA gene, multi-copy integration, gene silencing

LI Xu-Gang, CHEN Song-Biao, LU Zi-Xian, CHANG Tuan-Jie, ZENG Qian-Chun and ZHU Zhen. Impact of Copy Number on Transgene Expression In Tobacco[J]. J Integr Plant Biol., 2002, 44(1): 120-123.

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Impact of Copy Number on Transgene Expression In Tobacco

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