Abstract:

PSAG12-IPT gene was introduced into an elite rice ( Oryza sativa L. ssp. indica) restorer line Minghui 63 through Agrobacterium mediated transformation method. Out of 61 independent transgenic plants obtained, a few acquired a recognizable phenotype in which leave senescence was delayed to a great degree. The results of field plot test on two homozygous transgenic lines indicated: (1) the stay green ability of transgenic plants was significantly improved; (2) both the seed setting rate and the number of panicles per plant of transgenic plants were significantly increased compared with that of the non transgenic plants of Minghui 63; and (3) the plant height of transgenic plants was significantly reduced.

通过转P_SAG12-IPT基因培育延缓叶片衰老水稻
林拥军* 曹孟良** 徐才国 陈 浩 魏 君 张启发
（华中农业大学作物遗传改良国家重点实验室分子生物学分室，武汉430070)

摘要： 利用农杆菌介导的遗传转化方法将P_SAG12－IPT导入籼稻品种明恢63。在获得的61个独立的转基因植株中，有一些表现出叶片衰老显著延缓，对选择的两个纯合转基因株系的小区试验的结果显示：（1）转基因植株倒三叶的持性极显著延长；（2）两个纯合转基因株系比原品种的结实率极显著提高，株高极显著降低；（3）两个纯合转基因株系的有效穗数比原品种极显著或显著提高。

关键词： 水稻；农杆菌；转化；P_SAG12－IPT；持绿性；农艺性状

Key words: Oryza sativa, Agrobacterium, transformation, PSAG12-IPT, stay green, agronomic traits