J Integr Plant Biol. ›› 2002, Vol. 44 ›› Issue (11): 1384-1386.

• Research Articles • Previous Articles    

Antagonistical Mode of Pichia membranefaciens to Rhizopus stolonifer in Wounds of Peach Fruit

WAN Ya Kun, TIAN Shi Ping   

  • Published:2002-11-21

Abstract:

拮抗菌Pichia membranefaciens与病原菌Rhizopus stolonifer在桃果实伤口处的拮抗作用
万亚坤 田世平*

(中国科学院植物研究所光合作用与环境分子生理学重点实验室,北京100093)

摘要: 膜醭毕赤酵母(Pichia membranefaciens Hansen)是本实验室从果实上分离获得的一种能有效防治桃果实采后软腐病的拮抗菌.本文将P. membranefaciens与葡枝根霉(Rhizopus stolonifer)在桃果实伤口部位共培养24 h后,用扫描电子显微镜观测了它们的拮抗作用.结果表明,在有病原菌的地方聚集了大量的酵母拮抗菌,而且拮抗菌紧密地吸附在病原菌的菌丝体上.结合以前的研究结果可以推断,P. membranefaciens主要通过与病原菌进行营养和空间的竞争,紧密地吸附在病原菌菌丝体上分泌能降解病原菌细胞壁的水解酶(如几丁质酶和β-1,3-葡聚糖酶),并可能诱导寄主产生抗性,从而抑制桃软腐病的发生.

关键词: 拮抗作用;膜醭毕赤酵母;软腐病菌;扫描电镜

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Key words: antagonistical mode, Pichia membranefaciens, Rhizopus stolonifer, SEM

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