Abstract:

LK783 was found to be a good fertility restorer for K type male sterility of wheat (Triticum aestivum L.). RAPD and ISSR (inter simple sequence repeat polymorphism) markers were employed to map the major restoring gene in LK783. Maintainer and restorer DNA pools were established using the extreme sterile and fertile plants among KJ5418A//911289/LK783 F1 population, respectively. Four hundred and eighteen RAPD primers and 33 ISSR primers were used for screening polymorphisms between the two pools, and amplification bands using a RAPD primer of OPK18 and an ISSR primer of UBC 845 were found polymorphic between the two pools. Linkage analysis showed that OPK18 450 and UBC 845 800 were linked to the restoring gene in LK783. The distance between the restoring gene and OPK18450 was (15.07±6.28) cM (centiMorgan), with the distance between the restoring gene and UBC 845800 being (8.20±4.85) cM. The marker of UBC 845800 was located on chromosome 1B S by amplifying nulli tetrasomics and 1B ditelosomics of Chinese Spring with the primer of UBC 845, in dicating that the restoring gene in LK783 was located on 1BS. The breeding for new fertility restorer lines of K type cytoplasmic male sterility of wheat would be facilitated by using the two markers.

K型小麦细胞质雄性不育系育性恢复基因的RAPD和ISSR标记

刘保申^1,2  孙其信²  孙兰珍¹  高庆荣¹  解超杰²  窦秉德²  倪中福²  魏艳玲²  张延传¹

(1.山东农业大学农学系,泰安,271018；2.中国农业大学植物遗传育种系,北京,100094)

利用RAPD和ISSR分子标记对K型小麦(Triticum aestivum L.)雄性不育恢复系LK783的主效恢复基因进行了标记定位.以K冀5418A//911289/LK783三交F1分离群体的极端不育株和极端可育株分别建立保持池和恢复池,利用418个RAPD和33个ISSR引物对两池间的多态性进行了研究.分析表明RAPD引物OPK18和ISSR引物UBC-845在两池间扩增出稳定的多态性差异,在分离群体上的验证结果表明LK783的育性恢复基因与两个引物的扩增位点有连锁关系,在染色体上位于两个引物的扩增位点之间,与OPK18450的遗传距离为(15.07±6.28)cM (centiMorgan),与UBC-845800的遗传距离为(8.20±4.85)cM.这两个引物可应用于对育性恢复基因的标记辅助选择.最后,利用中国春缺体-四体系和双端体系进一步将UBC-845800定位于1BS, 表明LK783的育性恢复基因也位于1BS.

关键词 小麦 雄性不育 恢复基因 简单重复序列间多态性 RAPD