Abstract:

In cultivated rice (Oryza sativa L.), F1 pollen sterility is controlled by at least 6 loci of the F1 pollen sterility genes. To map S-b, one of loci, rice variety Taichung 65 (T65) carrying S-bj/S-bj and its near isogenic line TISL2 carrying S-bi/S-bi were used to develop the mapping population. One hundred and fifty-eight microsatellite markers were selected to survey T65 and TISL2. RM13 on chromosome 5 was found to be polymorphic between them. Cosegregation indicated that RM13 was closely linked with locus S-b. Eleven RFLP markers were selected on the corresponding region from the genetic map of Rice Genome Research Program (RGP) of Japan to convert into sequence tagged site (STS) markers. Amplicon length polymorphism (ALP) was carried out, but none of them was found to be polymorphic between T65 and TISL2. Then PCR- based RFLP (PBR) was done using six 4-nucleotide recognizing restriction endonucleases. Polymorphism was detected when PCR products of R830STS and R2213SSTS were digested with TaqⅠ. Genetic analysis indicated that the distance between locus S-b and markers R830STS, RM13 and R2213SSTS were 3.3 cM (centiMorgan), 5.2 cM and 5.5 cM, respectively. These PCR-based markers could be directly used in marker assisted selection. The technical systemcombining genetic mapping and PCR-based marker assisted selection will facilitate the development of molecular breeding.

栽培稻F1花粉不育基因座S-b的PCR标记定位

李文涛  曾瑞珍  张泽民  张桂权^*

(华南农业大学植物分子育种研究中心,广州,510642)

栽培稻(Oryza sativa L.)杂种F1花粉不育性至少由6个基因座位所控制.为了定位其中的一个基因座位S-b,选用了158个微卫星标记对粳型品种"台中65"及其近等基因系TISL2之间的多态性进行了分析.结果发现第5染色体短臂上的RM13在亲本间存在多态性.连锁分析表明,RM13与S-b座位紧密连锁.根据微卫星标记分析的结果,在RGP(Rice Genome Research Program of Japan)发表的遗传图谱的相应位置上选取了11个RFLP标记,利用这些标记的末端序列设计特异PCR引物,进行ALP (amplicon length polymorphism)分析,结果11对引物均没有ALP.用6种四碱基识别位点的限制性内切酶进行PBR(PCR-based RFLP)分析,发现由R2213S和R830两克隆设计的STS (sequence-tagged site)标记在T65与TISL2之间存在酶切多态性.R830STS、RM13和R2213SSTS与S-b座位的遗传距离分别为3.3 cM (centiMorgan)、5.2 cM和5.5 cM.这些以PCR为基础的分子标记可以直接应用于分子标记辅助选择中,从而建立了基因定位与分子标记辅助选择一体化的技术体系,使S-b座位在育种中可以得到有效的利用.

关键词 F1花粉不育性 基因定位 微卫星标记 序列标志位点(STS) 水稻 

通讯作者 Email: gqzhang@scau.edu.cn

Key words: F1 pollen sterility, genetic mapping, microsatellite, sequence-tagged site (STS), rice