J Integr Plant Biol. ›› 2002, Vol. 44 ›› Issue (5): 562-566.

• Research Articles • Previous Articles     Next Articles

Cloning and Expression Analysis of a Human Putative Tumor Suppressor Gene Homologue from Rice

GE Xiao Chun, ZONG Hui, ZHAN Shu Xuan, CHEN Ji Chao, SUN Chong Rong, CAO Kai Ming   

  • Published:2002-05-15

Abstract:

A gene homologous to the human putative tumor suppressor gene QM, designated OSQM1, was isolated from rice (Oryza sativa L.) genomic DNA library using through homology screening. It contained 4 exons and 3 introns, encoding a protein of 219 amino acids with 46 basic amino acids, leading to a high isoelectric point of 11.02. Homology search showed that this gene existed in eukaryotes and highly conserved throughout eukaryotes, suggesting an essential role of this gene. Northern blot analysis showed that it was expressed in various rice organs, but at lower level in developing flower and callus tissue than in other vegetative organs. Its expression levels in roots and leaves were influenced by different environmental factors.

水稻中一个人类肿瘤抑制基因同源物的克隆与表达分析
葛晓春* 宗 晖 詹树萱 陈继超 孙崇荣 曹凯鸣
(上海复旦大学生命科学学院生物化学系,上海200433)

摘要: 用同源筛选方法 ,从水稻 (OryzasativaL .)基因组文库中分离到一个与人类肿瘤抑制基因QM具有同源性的基因 ,命名为OSQM1。该基因包括 4个外显子和 3个内含子 ,编码 2 19个氨基酸 ,其中有 4 6个碱性氨基酸 ,其等电点高达 11.0 2。同源性搜寻发现此基因存在于真核生物中而且保守性较强 ,表明它可能具有重要的作用。North ern分析结果表明 ,它在不同的水稻器官中都有表达 ,但在花和愈伤组织中的表达水平明显低于其他营养器官。它在根和叶中的表达水平受环境因素的影响。

关键词: 肿瘤抑制基因;QM;真核生物

通讯作者。E-mail:gexiaochunl@ yahoo.com.cn.

Key words: tumor suppressor gene, QM, eukaryotes

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
备案号:京ICP备16067583号-22