Abstract:

The different resistance of cotton (Gossypium hirsutum L.) cultivars to crude toxin of Verticillium dahliae (VD) was correlated with the activities of chitinase and β-1, 3-glucanase in callus cells. The activities of chitinase and β-1, 3-glucanase in the callus cells treated with the VD-toxin were increased to the higher level at earlier time point in resistant cultivars than these in the susceptible cultivars. Exogenous salicylic acid (SA) induced the accumulation of chitinase and β-1,3-glucanase, which resulted in the resistance of callus cells to the VD. toxin. Western blot using a polyclonal antibody against β-1,3-glucanase identified 28 kD protein that was induced by VD-toxin, SA, or VD-toxin plus SA.

水杨酸和黄萎病菌毒素处理棉花愈伤组织使b-1,3-葡聚糖酶
和几丁质酶的活性增加
李颖章* 郑晓华 唐海林 朱建伟 杨晶明
(中国农业大学生物学院，植物生理生化国家重点实验室，北京 100094)

摘要： 不同品种棉花(Gossypium hirsutum L.)愈伤组织对黄萎病菌毒素粗提物的抗性与体内β-1,3- 葡聚糖酶和几丁质酶活性水平有关。在毒素处理下，抗性品种比感性品种酶活性增加的幅度大、时间早。外源水杨酸(SA)处理后，棉花愈伤组织中的β-1,3- 葡聚糖酶和几丁质酶活性增加。抗β-1,3- 葡聚糖酶多克隆抗体与28 kD 的蛋白条带有免疫交叉反应，毒素、SA、毒素+SA 均能诱导该蛋白条带出现。
关键词： 棉花愈伤组织;黄萎病菌毒素;水杨酸 β-1,3- 葡聚糖酶;几丁质酶