J Integr Plant Biol. ›› 2003, Vol. 45 ›› Issue (9): 1071-1075.

• Research Articles • Previous Articles     Next Articles

Expression of a Wheat S-like RNase (WRN1) cDNA During Natural- and Dark-induced Senescence

CHANG Sheng-He, YING Jia, ZHANG Ji-Jun, SU Jun-Ying, ZENG Ya-Juan,TONG Yi-Ping, LI Bin, LI Zhen-Sheng*   

Abstract:

An S-like RNase cDNA had been isolated from common wheat (Triticum aestivum L.). The transcription of WRN1 mRNA was down-regulated by natural- and dark-induced senescence. But it was not senile-tissue-specific. As the two key histidine residues were replaced, WRN1 may not be active as RNase. Southern blotting analysis showed that WRN1 exists as one of a small gene family in common wheat genome.

小麦类核糖核酸酶基因(WRN1)cDNA 在自然衰老和黑暗诱导衰老条件下的表达
常胜合  英 加  张吉军  苏俊英  曾雅娟  童依平  李 滨  李振声

(1.中国科学院遗传与发育生物学研究所,北京 100101;2.中国科学院生态环境研究中心,北京 100101)
摘要: 从普通小麦(Triticum aestivum L.)中分离了一个类核糖核酸酶(WRN1)基因的cDNA。WRN1的转录受自然衰老和黑暗诱导衰老的负调控。在幼嫩组织中WRN1也有表达。由于在两个保守的位置上组氨酸被替换,WRN1很可能已经失去了核糖核酸酶的活性。 Southern分析表明,在普通小麦基因组中,WRN1以一个小基因家族的形式存在。
关键词: 小麦类核糖核酸酶(WRN1);叶片衰老;普通小麦

通讯作者。E-mail:<zsli@genetics.ac.cn>。

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