Abstract: Two flavonoid glycosides separated from water soluble fraction of Oxytropis ochrocephala Bge. were identified by using IR, NMR, and MS spectroscopic methods. The aglycones and sugars of the two compounds were determined directly from the hydrolytic solutions by using a sugar column and a bonded phase column. The results showed that compound 1 contained two kinds of sugars which have the same retention times as galactose and glucose, compound 2 only contained galactose. The aglycones of the two compounds have the same retention times and UV-spectra of rhamnocitrin. Finally, the structures of the two compounds were elucidated as rhamnocitrin-3-galactoside-4’-glucoside and rhamnocitrin-3-galactoside. The first one is a new flavonoid glycoside and the second one is, for the first time, found in the Oxytropis species.

Key words: Oxytropis ochrocephala, Rhamnocitrin-3-galactoside-4’-glucoside, Rhamno-citrin-3-galactoside