Influence of Nitrogen Sources on Glutamine Synthetase in Wheat Seedling

Abstract

Abstract: Influence of nitrogen sources on the activity of glutamine synthetase (GS), GS isozymes and GS-mRNA in wheat (Triticum aestivum L. ) seedlings has been studied by DEAE-cellu- lose column chromatography, enzyme activity assay and Northern blot technique, and activity of GS and nitrate reductase (NR) was compared. The results indicated that the activity of GS in roots (GSr) and cytoplasmic GS (GS1) in both intact and excised leaves was higher with NH4+-N as the sole nitrogen source than with NO3--N. However, NO3- promated the activity of chloroplasmic GS (GS2) in leaves of wheat with NO3- -N as the sole nitrogen surce. On the level of transcription, NH+ raised the level of GS mRNA in roots, but NO3- raised the level of GS-mRNA in leaves of wheat seedling.

Key words: Glutamine synthetase, Nitrogen source, Glutamine synthetase-mRNA, Wheat

Huang Qin-ni, Yin Li-ping, Chai Xiao-qing, Liu Xiang-lin, Hong Jian-ming and Zhao Wei-ping. Influence of Nitrogen Sources on Glutamine Synthetase in Wheat Seedling[J]. J Integr Plant Biol., 1995, 37(11): -.

[1]	Shupeng Liu, Li Li, Wenlong Wang, Guangmin Xia and Shuwei Liu. TaSRO1 interacts with TaVP1 to modulate seed dormancy and pre-harvest sprouting resistance in wheat [J]. J Integr Plant Biol., 2024, 66(1): 36-53.
[2]	Jingyi Wang, Chaonan Li, Long Li, Lifeng Gao, Ge Hu, Yanfei Zhang, Matthew P. Reynolds, Xueyong Zhang, Jizeng Jia, Xinguo Mao and Ruilian Jing. DIW1 encoding a clade I PP2C phosphatase negatively regulates drought tolerance by de-phosphorylating TaSnRK1.1 in wheat [J]. J Integr Plant Biol., 2023, 65(8): 1918-1936.
[3]	Guoguo Lv, Yixiao Zhang, Lin Ma, Xiangning Yan, Mingjie Yuan, Jianhui Chen, Yongzhen Cheng, Xi Yang, Qi Qiao, Leilei Zhang, Mohsin Niaz, Xiaonan Sun, Qijun Zhang, Shaobin Zhong and Feng Chen. A cell wall invertase modulates resistance to fusarium crown rot and sharp eyespot in common wheat [J]. J Integr Plant Biol., 2023, 65(7): 1814-1825.
[4]	Qiang He, Dan Ma, Wei Li, Longsheng Xing, Hongyu Zhang, Yu Wang, Cailian Du, Xuanzhao Li, Zheng Jia, Xiuxiu Li, Jianan Liu, Ze Liu, Yuqing Miao, Rui Feng, Yang Lv, Meijia Wang, Hongwei Lu, Xiaochen Li, Yao Xiao, Ruyu Wang, Hanfei Liang, Qinghong Zhou, Lijun Zhang, Chengzhi Liang and Huilong Du. High-quality Fagopyrum esculentum genome provides insights into the flavonoid accumulation among different tissues and self-incompatibility [J]. J Integr Plant Biol., 2023, 65(6): 1423-1441.
[5]	Xuening Wei, Yuyan Li, Xiuliang Zhu, Xin Liu, Xingguo Ye, Miaoping Zhou and Zengyan Zhang. The GATA transcription factor TaGATA1 recruits demethylase TaELF6‐A1 and enhances seed dormancy in wheat by directly regulating TaABI5 [J]. J Integr Plant Biol., 2023, 65(5): 1262-1276.
[6]	Guanghui Yang, Man Feng, Kuohai Yu, Guangxian Cui, Yan Zhou, Lv Sun, Lulu Gao, Yumei Zhang, Huiru Peng, Yingyin Yao, Zhaorong Hu, Vincenzo Rossi, Ive De Smet, Zhongfu Ni, Qixin Sun and Mingming Xin. Paternally imprinted LATE-FLOWERING2 transcription factor contributes to paternal-excess interploidy hybridization barriers in wheat [J]. J Integr Plant Biol., 2023, 65(12): 2587-2603.
[7]	Lichao Zhang, Guoxiang Liu, Jizeng Jia, Guangyao Zhao, Chuan Xia, Lina Zhang, Fu Li, Qiang Zhang, Chunhao Dong, Shuangcheng Gao, Longzhi Han, Xiuping Guo, Xin Zhang, Jinxia Wu, Xu Liu, and Xiuying Kong. The wheat MYB-related transcription factor TaMYB72 promotes flowering in rice [J]. J Integr Plant Biol., 2016, 58(8): 701-704.
[8]	Salima Yousfi, Antonio J. Márquez, Marco Betti, José Luis Araus, and Maria Dolores Serret. Gene expression and physiological responses to salinity and water stress of contrasting durum wheat genotypes [J]. J Integr Plant Biol., 2016, 58(1): 48-66.
[9]	Liqing Gu, Bo Wei, Renchun Fan, Xu Jia, Xianping Wang and Xiangqi Zhang. Development, identification and utilization of introgression lines using Chinese endemic and synthetic wheat as donors [J]. J Integr Plant Biol., 2015, 57(8): 688-697.
[10]	Chengliang Sun, Lijuan Liu, Yan Yu, Wenjing Liu, Lingli Lu, Chongwei Jin, and Xianyong Lin. Nitric oxide alleviates aluminum-induced oxidative damage through regulating the ascorbate-glutathione cycle in roots of wheat [J]. J Integr Plant Biol., 2015, 57(6): 550-561.
[11]	Chihong Zhang, Lifeng Gao, Jiaqiang Sun, Jizeng Jia, and Zhenglong Ren. Haplotype variation of Green Revolution gene Rht-D1 during wheat domestication and improvement [J]. J Integr Plant Biol., 2014, 56(8): 774-780.
[12]	Pedro Carvalho, Sayed Azam-Ali, and M. John Foulkes. Quantifying relationships between rooting traits and water uptake under drought in Mediterranean barley and durum wheat [J]. J Integr Plant Biol., 2014, 56(5): 455-469.
[13]	Aixia Li, Wenlong Yang, Xueyuan Lou, Dongcheng Liu, Jiazhu Sun, Xiaoli Guo, Jing Wang, Yiwen Li, Kehui Zhan, Hong-Qing Ling, and Aimin Zhang. Novel Natural Allelic Variations at the Rht-1 Loci in Wheat [J]. J Integr Plant Biol., 2013, 55(11): 1026-1037.
[14]	Lifeng Zhao, Haoshan Zhang, Bingjing Zhang, Xiaojuan Bai and Chunjiang Zhou. Physiological and Molecular Changes of Detached Wheat Leaves in Responding to Various Treatments [J]. J Integr Plant Biol., 2012, 54(8): 567-576.
[15]	Adinda P. Derkx, Simon Orford, Simon Griffiths, M. John Foulkes, and Malcolm J. Hawkesford. Identification of Differentially Senescing Mutants of Wheat and Impacts on Yield, Biomass and Nitrogen Partitioning [J]. J Integr Plant Biol., 2012, 54(8): 555-566.

Influence of Nitrogen Sources on Glutamine Synthetase in Wheat Seedling

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments