J Integr Plant Biol. ›› 1994, Vol. 36 ›› Issue (12): -.

• Research Articles •    

Observation on Differentiation Potential and Chromosome Stability of Callus in Subcultures of Picea wilsonii

Yang Ying-gen, Gui Yao-lin, Tang Wei, Zhao Ran and Guo Zhong-chen   

Abstract: Embryogenic calli of Picea wilsonii Mast. cultured on modified 59 medium supplemented with 1 ppm 2, 4-D and 1 ppm Kt still remained high reproductive potential after sequential monthly subcultures for 3 years. When the callus were transferred to 1/2 strength modification 59 medium with 1 ppm ABA, a lot of somatic embryos were produced after culturing about 3 monthes. The frequency of callus embryogenesis was above 90 %. Chromosome counting showed that the chromosome ploidy of callus cells of P. wilsonii was stable in subcultures for 3 years. The chromosome number and karyotype formula is 2n= 24= 16m (6sc) +8sm+2B. This result agrees with that form root-tip squash of seedling.

Key words: Picea wilsonii, Somatic embryogenesis, Chromosome

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