Molecular Biological Identification of a Rice Salt Tolerant Line

Abstract

Abstract: A rice salt tolerant line No.170 was obtained from EMS treated anther culture of japonica variety and selected under salinity stress. The salt tolerance trait has been stably inherited through nine generations. In order to characterize this mutant line in molecular biological respects, 130 RFLP genetic markers along 12 pairs of chromosomes based on McCouch’s. rice RFLP linkage map were used to assay polymorphism between the mutant line and its original variety, was shown that allelic differences were found at two linked loci RG711 and RG4 on chromosome 7. Besides, proteins of roots and leaves between the mutant and the control plants were compared by means of two dimensional polyacrylamide gel electrophoresis. The comparison showed that some new proteins appeared in the salt tolerant plant under salinity-stress condition, which might be produced by salt inducible genes.

Key words: Rice, Salt tolerance mutation, RFLP, Two dimensional PAGE

Chen Shou-yi, Zhu Li-huang, Hong Jian and Chen Shao-lin. Molecular Biological Identification of a Rice Salt Tolerant Line[J]. J Integr Plant Biol., 1991, 33(8): -.

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Molecular Biological Identification of a Rice Salt Tolerant Line

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