Abstract: We report for the first time the use of polyethylene glycol 1540 as a freeze-fracture reagent on plant specimen prepared for observation by scanning electron microscopy. This method is a good fracture technique after being successfully experimented, which in some aspect is better than the “O-D-O” method.

Key words: Polythylene glycol 1540, Anther of Weigela florida, Scanning electron microscope, Freezing fracture