Abstract: Effects of age and variety of explant as well as of exogenous hormones on regeneration of style-stigma-like structure in vitro were observed in saffron. (1) Explants excised from stalk, perianth, anther, ovary, style and stigma showed different reactions in vitro, only young perianth and style could regenerate the style-stigma-like structures; (2) Age of the perianth explant had obvious effect on induction frequency, and the explants excised from an inflorescence of 24mm in length showed the highest frequency (37.5%) and (3) Experiments on exogenous hormones made clear that supplement with K 5mg/L and NAA 4mg/L was advantageous to regeneration of style-stigma-like structure. Induction frequency reached 35.3%. Supplement with 6-BAP 7mg/L and NAA 9mg/L could promote the formation of stigma like structures.

Key words: Crocus sativus L., Plant tissue and organ culture, Perianth, Style, Stigma