Abstract: Newly extended leaves of in vitro seedling of Actinidia eriantha Benth. were used for protoplast isolation. Protoplasts were cultured in liquid MS medium (devoid of NH4NO3) supplemented with 1.0 mg/L 2, 4-D and 0.4 mol/L glucose. The plating efficiency after 3 weeks of culture was about 19.4 %. Protoplasts-derived cells divided sustainably and developed into calli of 2 mm in size in the original protoplast-culture-medium without adding fresh medium so to decrease the osmotic pressure. These calli regenerated shoots when being transfered to MS medium with 0. 5 mg/L zeatin and 0. 1 mg/L IAA. Regenerated shoots were rooted by immersion in 20 ppm IBA solution before culturing on half-strength MS medium devoid of growth regulators.

Key words: Actinidia eriantha, Leaf protoplasts, Plantlet regeneration