J Integr Plant Biol. ›› 1996, Vol. 38 ›› Issue (8): -.

• Research Articles •    

Triploid Citrus Plants Obtained from Crossing the Diploids with Allotetraploid Somatic Hybrids

Deng Xiu-xin, Yin Hua-lin, Li Feng, Guo Wen-wu and Ye Wen-ming   

Abstract: Hybridizing the diploid monoembryonic pummelo (Citrus grandis) and polyembryonic tangerine (C. reticulata cv. Huanongbendizao) with allotetraploid somatic hybrids from protoplast fusion were conducted. Seeds of pollinated fruits were found to be abortive 90 days after pollination. The aborted seeds were then cultured on media of MT supplement with 1 mg/L GA3 or with 500 mg/L of malt extract. 25.6% of the seeds germinated or developed into embryoids: The entire plants were transplanted into soil after grafting shoots on the root-stocks of trifoliate orange in vitro, if the germinated embryos have poor roots or no root at all. A total of 73 plants from 6 different combinations were obtained, among which 20 were verified as triploids with 2n= 3x=27 chromosomes, 32 diploids 2n= 2x= 18, 8 a- neuploids and the rest 13 unconfirmed.

Key words: Citrus, Somatic hybrid, Sexual hybridization, Triploid plants

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