Abstract: An investigation of the chemical constituents of lipid of Tremella fuciformis Berk. is the central theme of this study. The sterols, fatty acids and phospholipids are separated by recrystallization, column chromatography and preparative thin layer chromatography, and their constituents are then identified by gas liquid chromatography and spectral data (UV, IR, MS and NMR). The results show that sterols comprise 16.8% ergosterol, 28.5% ergosta-5,7-dien-3β-ol and 54.7% ergost-7-en-3β-ol; fatty adds comprise 1.32%tmdeeanoic acid, 2.37% laurie acid, 1.28% tridecanoic acid, 0.09% myristic acid, 5.43% pentadecanoic acid, 17.20% palmitic acid, 3.11% stearic acid, 2.37% palmitoleic acid, 38.83% oleic acid and 27.98% linoleic acid; phospholipids comprise phosphatidylethanolamine, phosphatidylcholine phosphatidyl glycerol, phosphatidylserine and phosphati- dylinositol. Altogether there are seventeen constituents in the lipid.

Key words: Tremella fuciformis, ergosterol, ergosta-5,7-dien-3β-ol ergost-7-en-3β-ol