Abstract: The methods of isolation of leghemoglobin from alfalfa nodules and some characteristics of the proteins were described. It is indicated that the purified leghemoglobin massage contains precursor mRNA through in vitro translation of leghemoglobin mRNA and pulse chase experiment. The immunoprecipitation between leghemoglobin components from root nodules of legumes and IgGs against alfalfa leghemoglobins showed that it is possible to be of closer relationship among several kinds of legumes.