Abstract: The calli could be induced from the cotyledon in Miller medium supplemented with high concentration of kinetin (5–10 ppm) or with 2 ppm NAA plus 0.5 ppm kinetin. The callus induced by NAA plus kinetin was much different from that by kinetin alone. The former was loose and soft, while the later was tight and firm. About half a month after inoculation the later formed globe-like tissues and then new buds initiated and developed. The roots initiated from the buds when it was transplanted into Miller medium with low concentration of kinetin. The plantlets were thus obtained. However, the plantlet could not be gotten in white medium or Miller medium when hypocotyl explants were used. Therefore, it is concluded that regulation of callus formation and differentiation of the calli by exogenous hormone is closely related to some endogenous factors and external condition.