J Integr Plant Biol. ›› 1979, Vol. 21 ›› Issue (3): -.

• Research Articles •    

Cytohistological Studies on the Tissue Culture of Olea europaea L. II. Histogenesis and Organogenesis

Wang Kai-ji, Zhang Pi-fang, Ni De-xiang, Zhu Xiu-zhu and Yang Wei-qin Bao Zi-hua   

Abstract: After the calli derived from Olea europaea stem tissue have been introduced to the subculture on the solid medium for differentiation, a periderm was partially formed from the wound cambium in the outer region of the callus. At the same time, some scattered tracheids and vascular bundles were differentiated in the inside of the callus. These vascular bundles did not form a vascular system and also had no rela- tion to the organogenesis. In addition, there were some embryonic cells induced at random from parenchymatous cells in the callus, and these embryonic cells were characterized as the meristematic cells. Two types of meristematic tissues, namely, meristematic cellular mass and meristematic nodule, were produced by different types of mitotic division respectively. The meristematic nodules formed a growth center without any differentiation, but later, they were differentiated tracheids in the inner surrounded by the cambium-like cells. With monopolarity the root primordia were produced from this type of nodules. But the adventitious buds were derived from the meristematic cellular masses. Therefore, realize that the process of differentiation and dedifferentiation all occurs in the callus tissue. The structural differences among the nodules with tracheids, and the origin of buds and root primordia are also briefly discussed.

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
备案号:京ICP备16067583号-22