Abstract: The results for the quantitative analysis and identification of steroidal sapo-genins of 16 species of Dioscorea rhizomes are given in Table 1. The amount of steroidal sapogenin varies from 0.073%–5.93%, its highest content of diosgenin is 5.93% in D. zingiberensis C. H. Wright. 2. The results observed from paper chromatography, thin layer chromatography and gas-liquid chromatography revealed that except the sapogenin of D. chingii Pr. and Br. is similar to tokorogenin, all other sapogenins after recrystallization yield a spot identical to diosgenin or yamogenin. Meanwhile, in most Dioscorea sample, a spot identical to △ 3, 5-diene-25D-spirostane was shown by using thin layer chromatography and gas-liquid chromatography. (Table 2). 3. The results observed from infrared spectrum and gas-liquid chromatography revealed that the sapogenins of D. collettii, Hook. f; D. collettii. Hook, f. var. hypoglauca, Pei and Ting. and D. tokoro Makino. contained 25L-spirostane. (A900≤ A920 cm-1).