Abstract: Method for shoot tip culture of apple seedlings (five cultivars) and rootstocks (seven strains) in vitro is described, All of the cultures were proliferated either under the light or in the dark on the Murashige and Skoog medium containing benzyladenine (BA) (0.5 mg/l) and naphthaleneacetic acid (NAA) (0–0.1mg/l). Rapid multiplication of the shoots may be obtained in a short time. These shoots (except rootstock M. 26)Can be readily rooted in modified Start and Bruce medium containing indolebutyric acid (IBA) (0.5–1.0 mg/l) within two weeks. So far the viable plants from three varieties have been obtained.