Abstract: This paper briefly describes the characteristics of high-speed, liquid chromatography and reports some examaples on application in the researches of phytochemistry. Analyses of chemical components of five species of Chinese medicinal plants were carried out on a chromatographic instrument, which was built by our institute. They are: Camptotheca acuminata Decne., Euphorbpia helioscopia L., Salvia miltionrhiza Bge., Schisandra sphenanthera Rehd. et Wils., Daphne genkwa Sieb. et Zucc. The results of separation in the column of pellicular beads packing were compared with that of small porous particles (< 10 μm)packing.