J Integr Plant Biol. ›› 1965, Vol. 13 ›› Issue (1): -.

• Research Articles •    

The Formation of Adventitious Roots and Buds in the Petiole of Detached Leaf of Pelargonium Graveolens

C. L. Lee, Y. S. Hu and S. J. Liu   

Abstract: Median sized leaves of Pelargonium graveolens L'Her. after being excised from the middle of the petioles were planted in quartz sand or common sand in greenhouse. After one week in culture, calluses were formed aberrantly at the wound surface of the petioles of most of the detached leaves. By the second week, adventitious roots initiated somewhere in the callus close to the vascular tissue of the splitted base of the petiole. Adventitious buds also initiated from the callus, but they were not related to the vascular tissue in the origin. There were two different modes of initiation: one from the depth of the callus (endogenous) and the other near its surface (exogenous). Soon after, the distal end of the bud primordium became flattened (most of them became inverted triangular in outline) as was seen in the longitudinal section. Later, the middle part of the distal end became depressed and differentiated into the first leaf primordium and the apical meristem. As the young bud became further elongated, the leaf primordia were consecutively formed. Consequently the structure of the adventitious buds approached normal. The apical portion emerged from the sand was similar to the mature one in structure.

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