Abstract: The PAS reaction is applied in staining cellulose cell walls and starch grains in plant tissue preparations. Different kinds of fixatives used in combination with this method yielded equally good results. Various trials were made to obtain satisfactory results for PAS reaction in plant tissues. Procedure with 10 min in 0.5% KIO4 followed by 30 min. in Schiff's reagent gave a suitable coloration. Cellulose walls and starch grains were colored cherry red or reddish purple. Counterstaining may be used if desired. Iron-alum haematoxylin, methyl green and orange G were used as counterstains with satisfactory results. Advantage is taken of the fact that lengths of time required for acetylation of cellulose and that of starch are different. Thus starch and cellulose can be stained differentially. Several kinds of plant materials including root and shoot apexes, stems and cotyledons were used as examples to demonstrate the results of employing this method.