J Integr Plant Biol. ›› 1982, Vol. 24 ›› Issue (5): -.

• Research Articles •    

The Cultivation of Hybrid Cotton in Vitro to Overcome the Incompatibility of Hybridization between the Species

Zhang Sheng-zhang   

Abstract: 2—3 days after cross-pollination the hybrid ovules of cotton (Gossypium hirsutum×G. arboreum) were cultivated in vitro. The hybrid zygot in excised ovule started to divide, frequently passing through several stages to develop into cotyledonous embryos. The embryos were inducted into seedlings. The hybrid plants in blossom were obtained as a result. The histological study was done for ovules of self- pollination in vivo and of cross-pollination in vitro. The early abortion of endosperm in hybrid ovules was observed. In the prophase the nucellus was disintegrated slowly, and in the middle and terminal phase it disintegrated more rapidly. These were identified in hybrid F1. The size of corolla is intermadiate form between female and male. The violet-red spots at the base of petals are similar to male, while there are no such spots in female. None of the pollen can fertilize. Hybrid F1 is not fertile after self-pollination or backcross by the pollen of parent. The chromosome number in root tip Cell of hybrid F1 is 2n=3x=39.

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